- 别名:Endopeptidase K,Tritirachium alkaline proteinase
CUSAg (WUHAN HUAMEI BIOTECH CO.,LTD) has recently launched a batch of high- quality proteinase K. Our company's biochemical detection platform was used to characterize the self produced proteinase K and showing its high purity and enzyme activity. It is applied in the COVID-19 nucleic acid extraction kit and completely digest various nucleases and other proteins.It can prevent viral nucleic acid degradation and optimize extraction efficiency. Both liquid/lyophilized powder can be provided to meet the detection requirments of COVID-19/HCV/HBV.
- 纯度:90%±5% by SDS-PAGE
- 保存条件:The shelf life of the lyophilized form is 24 months at -20℃/-80℃, 18 months at 4℃, and 12 months at room temperature.
- 应用:Genomic Genomic DNA,RNA Purification, Gene diagnostic kit, ISH
- 来源:Tritirachium album Limber
- 活性:≥30 U/mg
- 活性定义:One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C.
- 热稳定性:Enzyme activity maintains above 95% when it store at 4℃ for 1 year
- 规格:100mg, 1g, 1kg
- 图 片:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.The purity and the molecular weight of CUSAg proteinase K were over 95% and 28.9 kDa by SDS-PAGE.
According to the absorbance A275 for the vertical axis, the different concentrations of tyrosine as the horizontal axis, the standard curve was drawn. Then, the enzyme activity was 30U/mg by calculating.
The proteinase K was stored at 4°C for one year. Enzymetic activities were asaayed every month during one year.As shown in Fig.3, it indicates that proteinase K is stable and enzyme activity is maintained more than 95%.
Activity measurements from were compared to from three other vendors. Proteinase K from CUSAg is better than other vendors.
Detect Nucleic acid residue by agarose gel electrophores
Detect DNase residue by agarose gel electrophores
Detect RNase residue by agarose gel electrophores