产品分类
  • 产品名称:

    蛋白酶K

  • 英文名称:

    Proteinase K

  • 所属类别:
    分子诊断
  • 货号:
    CSB-DP437A
  • 别名:
    Endopeptidase K,Tritirachium alkaline proteinase
  • 产品描述:

    CUSAg (WUHAN HUAMEI BIOTECH CO.,LTD) has recently launched a batch of high- quality proteinase K. Our company's biochemical detection platform was used to characterize  the self produced proteinase K and showing its high purity and enzyme activity. It is applied in the COVID-19 nucleic acid extraction kit and  completely digest various nucleases and other proteins.It can prevent  viral nucleic acid degradation and optimize extraction efficiency. Both liquid/lyophilized powder can be provided to meet the detection requirments of COVID-19/HCV/HBV.

  • 纯度:
    90%±5% by SDS-PAGE
  • 形态:
    Liquid
  • 保存条件:
    The shelf life of the lyophilized form is 24 months at -20℃/-80℃, 18 months at 4℃, and 12 months at room temperature.
  • 应用:
    Genomic Genomic DNA,RNA Purification, Gene diagnostic kit, ISH
  • 来源:
    Tritirachium album Limber
  • EC号:
    3.4.21.14
  • 活性:
    ≥30 U/mg
  • 活性定义:
    One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C.
  • 热稳定性:
    Enzyme activity maintains above 95% when it store at 4℃ for 1 year
  • 规格:
    100mg, 1g, 1kg
  • 图 片:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.The purity and the molecular weight of CUSAg proteinase K were over 95% and 28.9 kDa by SDS-PAGE.

    According to the absorbance A275 for the vertical axis, the different concentrations of tyrosine as the horizontal axis, the standard curve was drawn. Then, the enzyme activity was 30U/mg by calculating.

    The proteinase K was stored at 4°C for one year. Enzymetic activities were asaayed every month during one year.As shown in Fig.3, it indicates that proteinase K is stable and enzyme activity is maintained more than 95%.

    Activity measurements from were compared to from three other vendors. Proteinase K from CUSAg is better than other vendors.

    Detect Nucleic acid residue by agarose gel electrophores

    Detect DNase residue by agarose gel electrophores

    Detect RNase residue by agarose gel electrophores